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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 432
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Cell_line_name |
8-MG-BA
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Brief_description |
confirmed as human with IEF of AST, LDH established from the frontal brain lobe of a
54-year-old woman with glioblastoma multiforme in 1989 (pre-treatment)
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Description |
human glioma established from the frontal brain lobe of a 54-year-old woman with
glioblastoma multiforme in 1989 (pre-treatment) confirmed as human with IEF of AST,
LDH
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Depositor |
Dr. A. Perzelova, Comenius University, Bratislava, Slovakia
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Morphology |
epithelial-like, adherent cells growing as monolayer
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Culture_conditions |
80-85% MEM (with Earle's salts) + 15-20% FBS split confluent culture 1:3 to 1:4
once a week using trypsin/EDTA; seed out at ca. 1 x 106 cells/25 cm 2 ; optimal
density at ca. 5-10 x 106 /175 cm 2 at 37C with 5% CO2 cell harvest of maximal
15 x 106 /175 cm 2
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Viruses |
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -,
SMRV -
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Properties |
cytokeratin (+), cytokeratin-7 -, cytokeratin-8( +), cytokeratin-17 -, cytokeratin-18 -,
desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule
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Doubling_time |
ca. 100 hours
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Mycoplasma |
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI,
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Fingerprint |
multiplex PCR of minisatellite markers revealed a unique DNA profile
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Cytogenetics |
human flat-moded hyperdiploid karyotype with 15% polyploidy - 52(47-52)<2n>X, -X,
+1, +1, +3, +5, +6, +7, +8, -19, +2-4mar, add(1)(p34-36;hsr), add(1)(p34-36),
der(1)t(1;17)(q11-12;q12-21), der(3;5)(q10;q10), i(5p), del(6)(q24-25), del(7)(p14),
i(7p), i(8q), add(8)(p21)x1-2, del(9)(q32q34)/add(9)(q32), add(11)(?q13),
del(12)(q14), del(18)(q22.2), add(19)(p13.1) - sideline with del(3)(p11),
der(6;9)(p10;q10) - 2-4 acf present in 31% cells
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Bibliographic_reference |
Perzelova et al., Neoplasma, 45: 53 (1998) [PMID: 9604998
] |
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